The Clinical Value of CEA mRNA、CEA Protein of Peripheral Blood in the Prediction of Colorectal Cancer
- VernacularTitle:外周血CEAmRNA、CEA检测在结直肠癌诊断中的临床价值
- Author:
Yun SHI
;
Zhenjun WANG
;
Bin WANG
- Publication Type:Journal Article
- Keywords:
Colorectal cancer;
Micrometastasis;
Carcinoembryonic antigen;
Reverse transcription polymerase chain reaction
- From:
Journal of Chinese Physician
2002;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Colorectal cancer is one of the most common malignancies with high incidence rate. Due to the progress made in diagnostic and therapeutic techniques, even about 30%～50% of patients with no evidence of metastasis on the time of diagnosis have recurrences. The present study is to investigate the carcinoembryonic antigen(CEA) mRNA,CEA protein expression in blood and to evaluate its clinical significance. Methods Peripheral blood samples were collected from 60 patients with colorectal cancer and 40 patients with colorectal polyp and 40 healthy volunteers. CEA mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR) and serum CEA was detected by Enzyme-linked immunosorbent assay. Results The positive rate of CEA mRNA expression in blood samples of 60 patients with colorectal cancer was 68.3%. And one of the healthy volunteers was positive. But three of the 40 patients with colorectal polyp(7.5%) were positive. The positive rate increased with the pathological staging and liver metastasis, but there were no significant relations between the rate and the malignant extent of cell differentiation. Conclusion The expression of CEA mRNA in peripheral blood cells correlates with the pathological staging and liver metastasis of colorectal cancer, and it may be one of potential uses to monitor the micrometastases of tumor. In order to discover the precancerous change,we can detect CEA mRNA expression in blood with polyp of colon.
