The Effects of Glucose on Gene Expression and Activity of Aldose Reductase in Endothelial Cells
- VernacularTitle:葡萄糖对内皮细胞醛糖还原酶基因表达及活性的影响
- Author:
Ling HE
- Publication Type:Journal Article
- Keywords:
Glucose;
Endothelial cells;
Aldose reductase;
Gene;
Activity
- From:
Journal of Chinese Physician
2002;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of glucose on gene expression and activity of aldose reductase (AR) in endothelial cells. Methods Human umbilical vein endothelial cells (HUVECs) were cultured in vitro. The activity of AR gene expression in mRNA level in HUVECs were measured by RT-PCR and biochemical assay. Results The AR mRNA levels and its activity increased in dose-depended manner in endothelial cells incubated for 24h with 11mmol/L,22mmol/L glucose, compared with those in 5.5 mmol/L glucose ( P
