Determination of the Pharmacokinetics Parameters of Recombinant Staphylokinase in Thrombosis Model of the Femoral Artery in Rabbits by Biological Assay
- VernacularTitle:生物法测定重组葡激酶在股动脉血栓形成模型兔中的药动学参数
- Author:
Weiqing WANG
;
Zhigao SHAO
;
Guangyu LIU
;
Hongwen ZHANG
;
Jing ZHANG
;
Jie FANG
;
Chunjian LI
- Publication Type:Journal Article
- Keywords:
Recombinant staphylokinase;
Animal model;
Femoral-artery thrombus;
Rabbits;
Plasma concentration;
Phar?macokinetics
- From:
China Pharmacy
2005;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the pharmacokinetics of recombinant staphylokinase(r-SAK)in a thrombosis model of femoral artery in rabbits.METHODS:Large glass plates were used for modified agar-well diffusion assay to measure r-SAK concentration in plasma of rabbits which had been administered different doses drug by different means respective?ly.RERULTS:The pharmacokinetics of r-SAK infusion in thrombosis model of the femoral artery in rabbits fits two-com?partment model,the plasma levels and the diameter of lytic circles showed a good linear correlation under the scope of20～2500IU/ml(r=0.9960),and the averaged recovery rate was(96.05?9.20)%.The peak concentrations of low,medial and high dose drop group and single iv group are(2.28?1.06),(3.54?0.32),(6.12?1.61)and(5.16?1.02)?g/ml.CONCLUSION:The biological assays is a simple,reliable method to determine the plasma level.
