Content Determination of2Components in Xiaozhiyin by HPLC Simultaneonsly
- VernacularTitle:高效液相色谱法同时测定消脂饮中2组分的含量
- Author:
Guoxi YU
;
Suyan CHEN
- Publication Type:Journal Article
- Keywords:
High performance liquid chromatography;
Salvianolic acid B;
Chrysophenine glycoside;
Content
- From:
China Pharmacy
2005;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a HPLC method for content determination of salvianolic acid B and chrysophenine glycoside in Xiaozhiyin.METHODS:The compounds were base-isolated on the column of Kromesile C 18 at30℃and gradient elution was performed in mobile phase of acetonitrile-0.15%phosphoric acid solution,the wavelength was320nm,and the flow rate was1ml/min.RESULTS:The linearity was obtained between sample size and peak area score over the range of0.8336?g～ 8.336?g(r=1.0000)for salvianolic acid B,and0.352?g～3.52?g(r=1.0000)for chrysophenine glycoside.The average re?covery rate was99.5%(RSD=0.40%)for salvianolic acid B and100.2%(RSD=0.70%)for chrysophenine glycoside.CON?CLUSION:The method is accurate,simple,and highly reproducible,and it can be used for the quality control of Xiaozhiyin.
