The effects of regional infusion of hypothermic captopril on the free radicals and MPO in rabbit spinal cord tissue of ischemia-reperfusion
- VernacularTitle:低温卡托普利局部灌注对缺血再灌注后脊髓组织自由基和MPO的影响
- Author:
Wei DU
;
Jianguo HU
;
Qiuxia HUANG
- Publication Type:Journal Article
- Keywords:
Spinal cord;
Ischemia/reperfusion injury;
Free radicals;
Captopril
- From:
Journal of Chinese Physician
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of regional infusion of hypothermic normal saline contained captopril on free radicals level and myeloperoxidase(MPO) activity in rabbits during spinal cord ischemia-reperfusion(I/R).Methods The 80 rabbits were randomly divided into four equal groups,group A(sham operated,no I-R injury,n=8),group B(control,only I/R,n=24),group C(I/R + intraaortic hypothermic normal saline,n=24),and group D(I/R + N.S plus 4mg/kg captopril,n=24).The model of spinal cord ischemia in the rabbit was induced with the aorta clamped.Hypothermic solution was infused into the isolated aortic segments.The content of spinal cord MDA and the activities of superoxide dismutase(SOD),MPO were measured at 8h,24h and 72 h after the reperfusion respectively.Results The level of spinal cord MDA increased,the activity of SOD decreased and the activity of MPO increased markedly after the reperfusion.Compared with group B,the MDA content decreased significantly at the three observation time points and the MPO activity decreased significantly in groups C and D(all P
