The effects of hypoxia inducible factor-2? on the proliferation and invasion of the hepatocellular carcinoma cells
- VernacularTitle:缺氧诱导因子对肝癌细胞生长和侵袭能力的影响
- Author:
Jian WANG
;
Jilin YI
;
Congjun WANG
- Publication Type:Journal Article
- Keywords:
Hypoxia inducible factor-2?;
Oligonucleotides;
hepatocellular carcinoma;
Proliferation;
Adhesion;
Invasion
- From:
Journal of Chinese Physician
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of hypoxia inducible factor-2?(HIF-2?) on the proliferation and invasion of hepatocellular carcinoma cell line SMMC-7721 under the anoxia condition.Methods Lipofectamine-mediated HIF-2? oligodeoxynucleotide(ODN) was transfected into SMMC-7721 cells and the expressions of HIF-2? mRNA and protein were detected to evaluate the transfection.The proliferative and adhesive activity was determined by methyl thiazolyl tetrazolium(MTT) method and the invasive activity was determined by a transwell cell culture chamber method.Results The expressions of HIF-2? mRNA and protein were inhibited significantly by HIF-2? antisense oligodeoxyribonucleotide(ASODN)(P0.05).Conclusion The blockage of HIF-2? expression inhibits the adhesive and invasive activity of SMMC-7721cells,and has no evident impact on their proliferation.
