Effects of high energy shock wave on oxidative stress and apoptosis in the renal cells of rabbits
- VernacularTitle:高能冲击波致肾细胞凋亡和氧化应激损伤的相关性研究
- Author:
Xiqing GUI
;
Jianping HUANG
;
Hua ZHANG
- Publication Type:Journal Article
- Keywords:
High energy shock wave;
Renal cell;
Apoptosis;
Oxidative stress;
Allopurinol
- From:
Journal of Chinese Physician
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between oxidative stress and cell apoptosis in the kidney of rabbit induced by high energy shock wave(HESW).Methods Forty healthy male rabbits were surgically managed to the mono-nephron models and randomized into 4 groups consisting of ten each: control group,1 000 HESW group,2 000 HESW group and allopurinol pretreated plus 2 000 HESW group.The rabbit kidney tissues were obtained.The activity of superoxide dismutase(SOD) and malondialdehyde(MDA) levels in the renal tissue were measured 24 hours after HESW application.The cell apoptosis was detected by TdT-mediated dUTP nick end labelling(TUNEL) and flow cytometry.Results Compared with the control group,the MDA level and the rate of apoptosis in the HESW groups increased,and the activity of SOD reduced significantly(P
