The protection of panax notoginseng saponins against ischemia reperfusion injury by liver graft in rats
- VernacularTitle:三七总皂甙对大鼠移植肝缺血再灌注损伤的保护作用
- Author:
Feng HAN
;
Jinxue ZHOU
;
Lin ZHANG
- Publication Type:Journal Article
- Keywords:
Liver transplantation;
Ischemia reperfusion;
NF-?B;
Intercellular adhesion molecule-1;
Panax notoginseng saponins
- From:
Journal of Chinese Physician
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protection of panax notoginseng saponins(PNS) against ischemia reperfusion injury by liver graft in rats.Method The experimental rat model of orthotopic liver transplantation was set up according to Kamada's technique and the rats were randomly divided into three groups: normal saline control group(group N),PNS preconditioning group(group P) and sham-operated groups(group S).The expression levels of NF-?B and intercellular adhesion molecule-1(ICAM-1) were detected by immunohistochemistry and the filtration of polymorphonuclear neutrophils(PMNs) was measured with myeloperoxidase(MPO) enzyme method in 2h,6h and 24h after ischemia reperfusion respectively.The activities of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) in the serum were also detected.Result The activation of NF-?B in group N increased significantly at 2h after reperfusion and then decreased.The expression level of ICAM-1 increased significantly at 2h after reperfusion,and reached its peak level at 6h in group N.The expression level of ICAM-1 was significantly lower in group P than that in group N(P
