Concentration Determination of Floxuridine in Serum and Organs of Mice by RP-HPLC
- VernacularTitle:反相高效液相色谱法测定小鼠血清及脏器中氟尿苷的浓度
- Author:
Jiafang LIAN
;
Sanqi ZHANG
;
Yi GU
;
Kunquan FANG
;
Miaomiao XI
;
Li WANG
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Floxuridine;
Serum;
Tissue homogenate
- From:
China Pharmacy
2005;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish RP-HPLC method for the fast determination of floxuridinein in serum and homogenate of some of the organs of mice. METHODS: The determination was performed on C18 chromatographic column with its mobile phase consisted of phosphate buffer(pH= 7.4) - methanol(95∶5) and with its detection wavelength at 268nm. RESULTS: Linear detection concentration ranges of floxuridinein were all at 15～240?g/ml. The minimum detection concentration was 3?g/mL(S/N≥3). The average extraction recovery was above 95%. The average recovery ranged from 91%to 112%. Both the intra-day and inter-day precisions were less than 10%. CONCLUSION: The method is simple, rapid, reliable and economical, and it can be used for the determination of serum floxuridinein concentration and the study of its body distribution in animals.
