Simultaneous Determination of 2 Constituents in Compound Chloramphenicol Auristillae by HPLC
- VernacularTitle:高效液相色谱法同时测定复方氯霉素滴耳液中2组分的含量
- Author:
Shunlin XIAO
;
Hao ZHANG
;
Yun YE
;
Bimin FENG
- Publication Type:Journal Article
- Keywords:
HPLC;
Compound chloramphenicol auristillae;
Chloramphenicol;
Furacillin;
Contents
- From:
China Pharmacy
2005;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a HPLC method for the determination of both chloramphenicol and furacillin in compound chloramphenicol auristillae.METHODS:The determination was performed on Tianhe Kromasil C18 column,the mobile phase consisted of methanol-water(60∶40) with flow speed at 1.0ml/min,detection wavelength at 278nm,column temperature at 25℃,and sample size at 20?l.The dexamethasone was used as internal standard.RESULTS:The linear ranges for chloramphenicol and furacillin were 31.75～127?g/ml and 26.25～105?g/ml,respectively;The average recovery rates were 100.4%(RSD=0.89%) and 100.7%(RSD=1.20%),respectively.CONCLUSION:This method is simple in operation,accurate and reliable in determination results and it can be used for the quality control of compound chloramphenicol auristillae.
