Inhibition of Telomerase Activity and Induction of Cell Apoptosis in Cutaneous T-cell Lymphoma Cell Line by Human Telomerase Reverse Transcriptase Gene Antisense Oligodeoxynucleotide
- VernacularTitle:hTERT反义寡核苷酸抑制Hut78细胞端粒酶活性及诱导细胞凋亡的研究
- Author:
Xiulian XU
;
Jinliang QI
;
Shuxian SHANG
;
Shengli CHEN
;
Xuesi ZENG
;
Jianfang SUN
- Publication Type:Journal Article
- Keywords:
Lymphoma, T-cell, cutaneous;
Oligonucleotides,antisense;
Telomerase
- From:
Chinese Journal of Dermatology
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of human telomerase reverse transcriptase (hTERT) gene antisense oligodeoxynucleotide (AODN) on telomerase activity and cell apoptosis in a cutaneous T-cell lymphoma (CTCL) cell line, Hut78. Methods Different concentrations (10 ?mol/L, 20 ?mol/L, 30 ?mol/L) of telomerase antisense oligodeoxynucleotide were introduced into Hut78 cells by lipofectamine-mediated DNA transfection technique. The expressions of hTERT mRNA and telomerase activity were assessed by reverse transcription-polymerase chain reaction and telomeric repeat amplification protocol, respectively. The proliferation and apoptosis of Hut78 cells were detected by flow cytometry. Results After 72 h of incubation, AODN down-regulated the expression of hTERT mRNA, inhibited telomerase activity significantly,and suppressed the viability of Hut78 cells in a time-dependent manner.Cell growth was most clearly suppressed with 30 ?mol/L of AODN after 72 h of incubation. The apoptotic rate was 13.05%. Conclusion Telomerase antisense oligodeoxynucleotide could suppress the viability and proliferation of CTCL cell line by inducing apoptosis of these cells.
