The Specific Immune Response Elicited by Recombinant CRT120/HPV6bE7 DNA Vaccine in Mice
- VernacularTitle:钙网蛋白基因120片段和人乳头瘤病毒6bE7基因诱导的小鼠细胞免疫反应
- Author:
Yan XU
;
Hao CHENG
;
Kejia ZHAO
;
Jun YE
;
Xing ZHANG
;
Tao SONG
;
Minli CHEN
;
Qi WANG
- Publication Type:Journal Article
- Keywords:
Papillomavirus, human;
Vaccines, DNA;
Calreticulin
- From:
Chinese Journal of Dermatology
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the cellular immune response to the recombinant DNA vaccine CRT120/HPV6bE7 in mice. Methods The recombinant encoding HPV6bE7, linked with CRT120, was constructed in pcDNA3.1 eukaryotic vector with the report gene GFP. The pcDNA3.1-GFP -HPV6bE7 was transfected into B16 cells by a lipofectamine kit. The C57BL/6 mice were vaccinated with recombinant DNA plamids. The T-cell phenotype in the peripheral blood lymphocytes of the immunized mice was measured by flow cytometry. The CTL activity of the lymphocytes from the spleens and lymph nodes, and the levels of IL-2 and IFN-? were analyzed. Results The constructed recombinant plasmid CRT120/HPV6bE7 was analyzed. Positive transfected cell clones were established and could stably express the target gene HPV6bE7. Compared with HPV6bE7, CRT120/HPV6bE7 plasmid enhanced to a greater extent CD8+ T-lymphocyte differentiation, the number of TCR?? T-lymphocytes and the levels of IL-2 and IFN-? (all P
