Content Determination of Matrine in It's Liposomes by Acid Dye Colorimetry
- VernacularTitle:酸性染料比色法测定苦参碱脂质体中苦参碱的含量
- Author:
Wenying WU
;
Kanghuai ZHANG
;
Yali CHEN
;
Xiaoxia CHE
;
Li XIANG
- Publication Type:Journal Article
- Keywords:
Acid dye colorimetry;
Matrine;
Liposomes;
Content determination
- From:
China Pharmacy
2005;0(22):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for content determination of matrine in it's liposomes by acid dye colorime_ try.METHODS:Sephadex gel G-50 column was established by swelling of matrine liposomes sample in distilled water for at least 12 hours,distilled water was used as mobile phase,after eluting,the column was mixed with 0.0 125%of bromothymol bl_ ue buffer solution and chloroform,and then the mixture was shaken,standing and demixed,the absorbability of chloroform layer was detected at the wavelength of 413 nm.RESULTS:The detection concentration of matrine showed good linearity with its absorbability within the range of 0.04～0.20mg/ml(r=0.9 972),the average recovery was 94.10%(RSD=1.86%).CONCL_ USION:The established method is simple and accurate,which can be used for quality control of matrine liposomes.
