Experimental study of the effect of platelet-activating factor acetylhydrolase on severe acute pancreatitis
- VernacularTitle:血小板活化因子乙酰水解酶对重症急性胰腺炎影响的实验研究
- Author:
Gang WANG
;
Bei SUN
;
Qinghui MENG
;
Hongchi JIANG
- Publication Type:Journal Article
- Keywords:
Plaelet Acivating Factor;
Severe Acute Pancreatitis
- From:
Chinese Journal of General Surgery
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of platelet-activating factor acetylhydrolase(PAF-AH) on(severe) acute pancreatitis(SAP).Methods Forty-five male Wistar rats were randomly divided into three groups(n=15 in each group),including group A(sham operation group),group B(SAP group) and group C(PAF-AH treated group).PAF-AH(5mg/kg) was infused through the dorsal artery of penis after 1 hour and 24 hours of establishing the model in group C.Forty-eight hours after animal models were established,the levels of maleic dialdehyde(MDA),thromboxane B2(TXB_2),prostaglandin I2(PGI_2),TXB_2/PGI_2,(platelet)-activating factor(PAF),endotoxin,tumor necrosis factor(TNF-?) in serum,and the activity of(myeloperoxidase)(MPO) in lung tissue were examined in each group.The pancreas was scored on the basis of pathological changes.Results Compared with group B,the levels of MDA,TXB_2,TXB_2/PGI_2,PAF,(endotoxin),TNF-? in serum,and MPO activity in lung tissue and the pathological scores of pancreas were(significantly) lower in group C(P0.05).Conclusions PAF plays a critical role during the course of SAP.(Administration) of PAF-AH can hydrolyze PAF,improve pancreatic microcirculatory dysfunction,suppress(systemic) inflammatory reaction,relieve pancreatitis-associated intestinal tract injury and lung injury and(markedly) ameliorate the state of SAP.
