Dual-direction effect of crenulatin on apoptosis of cerebral microvascular endothelial cells and it's mechanism
- VernacularTitle:大花红天素对脑微血管内皮细胞凋亡的双相调节作用及机制(英文)
- Author:
Ruizhe QIAN
;
Guoping ZHANG
;
Huiming JIN
;
Wenjian WANG
;
Fei YUE
;
Lianguo SHI
;
Xiaoyi QU
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Crenulation;
bEnd 3 cells
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
] AIM: To study the effect and the mechanism of crenulatin, an effective constituent of Chinese traditional medicine, on apoptosis of cerebral microvascular endothelial cells. METHODS: The following terminal concentrations of crenulatin were used in the study: 25 mg/L and 100 mg/L. Apoptosis of mouse cerebral microvascular endothelial cells (bEnd.3 cell line) was evaluated by flow cytometer, immunocytochemical assay (Fas, Bcl-2) and Western blotting (caspase-3) after culture for 24 h. RESULTS: Compared with control group, apoptosis of bEnd.3 cells in 25 mg/L group was significantly inhibited (P
