Expression of NADPH oxidase subunit p22phox in myocardial infarction rats
- VernacularTitle:大鼠心肌梗死后心肌NADPH氧化酶亚单位p22phox的表达
- Author:
Zhihong ZHAO
;
Xiaofeng BAO
;
Jiang SHAN
;
Geng XU
;
Guosheng FU
;
Meixiang XIANG
;
Xiaoye ZHEN
- Publication Type:Journal Article
- Keywords:
Ventricular remodeling;
NADPH oxidase;
Myocardial infarction
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To determine the relevance of NADPH oxidase subunit p22hox and the expression of superoxide anion on ventricular remodeling in myocardial infarction (MI) rats. METHODS: MI of Sprague-Dawley rats were established by left anterior descenting coronary artery ligation. 8 weeks after MI, Doppler echocardiography, hemodynamic study and histomorphometry were performed to analyze the ventricular remodeling. The level of thiobarbituric acid reactive substance in plasma and myocardium were measured, and the distribution of superoxide anion was observed with laser scanning confocal microscope. The expression of p22phox mRNA and protein level was detected by RT-PCR and immunohistochemistry. RESULTS: The left ventricular remodeling was significant in MI rats, also the level of thiobarbituric acid reactive substance increased in the plasma and non-infarcted myocardium. The expressions of p22-phox mRNA and protein levels, and superoxide anion increased in infarcted and non-infarcted myocardium in MI rats. CONCLUSION: Our results suggest that the expression of NADPH oxidase and its derived superoxide anion may take part in left ventricular remodeling through oxidative stresss after MI.
