Effect of chronic atrial fibrillation on Ca~(2+)/calmodulin dependent protein kinase Ⅱ expression in human atrial myocytes
- VernacularTitle:慢性心房颤动对人心房肌钙/钙调素依赖性蛋白激酶Ⅱ表达的影响
- Author:
Jinjin CHEN
;
Yingbin XIAO
;
Jian LIU
- Publication Type:Journal Article
- Keywords:
Atrial fibrillation;
Calcium overload;
Myocardium
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of chronic atrial fibrillation (AF) on free calcium concentration and expression of Ca 2+ /calmodulin dependent protein kinase Ⅱ (CaMKⅡ) in human atrial myocytes. METHODS: The intracellular free calcium concentration in acute isolated atrial myocytes and the expression of CaMKⅡ in atrial tissue of rheumatic heart disease patients with atrial fibrillation (AF) and with normal sinus rhythm were measured by laser scanning cofocal microscopy technique and Western blotting, respectively. RESULTS: The intracellular Ca 2+ concentration in patients with atrial fibrillation was significantly higher than that in patients with normal sinus rhythm [(276.38?38.12) nmol/L vs (122.28?45.63) nmol/L, P
