Effects of hypoxia on levels of nitric oxide, endothelin-1 and inducible nitric oxide synthase mRNA expression in cultured human umbilical vein endothelial cells
- VernacularTitle:低氧对人脐静脉内皮细胞一氧化氮和内皮素-1的生成及诱导型一氧化氮合酶mRNA表达的影响
- Author:
Xiaolan HU
;
Shuxin LIN
;
Daihua YU
;
Shifan ZHANG
- Publication Type:Journal Article
- Keywords:
Anoxia;
Vein;
Nitric oxide;
Endothelin-1;
Gene expression
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the effects of hypoxia on the levels of nitric oxide (NO), endothelin (ET-1) and the expression of inducible nitric oxide synthase (iNOS) mRNA in human umbilical vein endothelial cells (HUVECs), and further investigate the mechanism of hypoxic pulmonary hypertension. METHODS: On the basis of the HUVECs culture model, the methods of nitrate reductase and radioimmunoassay were used to determine the changes of NO and ET-1 in the medium secreted by HUVECs, and the expression of iNOS mRNA was analyzed by semi quantitative RT-PCR after exposure to hypoxia (3% O_2) for 6, 12 or 24 h. RESULTS: The contents of NO_2-/NO_3-and ET-1 in hypoxia group in the medium was significantly higher than that in control group at different time points (P
