Hindlimb ischemia and granulocyte colony-stimulating factor accelerates mobilization of endothelial progenitor cells in mice peripheral blood
- VernacularTitle:下肢缺血及G-CSF促进小鼠外周血内皮祖细胞动员
- Author:
Yinpin ZHOU
;
Lan HUANG
;
Yaoming SONG
;
Jun JIN
;
Xiaojing WU
;
Bin CUI
- Publication Type:Journal Article
- Keywords:
Ischemia;
Granulocyte colony-stimulating factor;
Endothelial progenitor cells
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To evaluate the mobilization of endothelial progenitor cells (EPCs) in mice peripheral blood during hindlimb ischemia alone or in combination with granulocyte colony stimulating factor (G-CSF). METHODS: Hindlimb ischemia was established in mice by surgical excision of both femoral arteries. Fluorescence-activated cell sorting (FACS) was used to detect the expression of cell-surface CD34 and vascular endothelial growth factor recepter-2 (VEGFR2) antigens. The ratio of double-positive cells for CD34 and VEGFR2 was regarded as the level of EPCs in peripheral blood. In G-CSF administration in combination with hindlimb ischemia group, the percentage of double-positive cells was also detected. RESULTS: As compared with control group, hindlimb ischemia increased the percentage of EPCs in mice peripheral blood. The hindlimb ischemia combined with G-CSF administration significantly enhanced the percentage of EPCs. CONCLUSION: Ischemia increases the number of EPC in peripheral blood. It may induce the migration of EPC from barrow to peripheral blood. By mobilizing barrow, G-CSF enhances this effect.
