Effect of fluvastatin on migration of vascular smooth muscle cells from rats
- VernacularTitle:氟伐他汀对大鼠血管平滑肌细胞迁移的影响及其机制
- Author:
Ying HAN
;
Liangdi XIE
;
Changsheng XU
;
Huajun WANG
- Publication Type:Journal Article
- Keywords:
Fluvastatin;
Platelet-derived growth factor;
Endothelin-1;
Cell movement;
Calcium
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect and mechanism of fluvastatin on the migration induced by platelet derived growth factor-BB (PDGF-BB) and endothelin-1 (ET-1) in cultured vascular smooth muscle cells (VSMCs). METHODS: Cultured VSMCs derived from spontaneously hypertensive rats (SHR) were used. Cell migration was determined by modified Boyden chamber assays. Intracellular free calcium ([Ca 2+ ]i) was measured with fluorescent Ca 2+ indicator Fura-2/AM. RESULTS: PDGF-BB and ET-1 significantly induced VSMCs migration, which was inhibited by pretreatment of VSMCs with fluvastatin (10 -9 -10 -5 mol/L) in a dose-dependent manner, and the peak inhibition rate of migration induced by PDGF-BB and ET-1 was over 86.67%. Fluvastatin also attenuated the increase in [Ca 2+ ]i induced by PDGF-BB and ET-1, with a peak inhibition rate of 86.76% and 65.32%, respectively. CONCLUSION: PDGF-BB and ET-1 promote migration of VSMCs from SHR.Fluvastatin may have direct inhibitory effects on cell migration induced by PDGF-BB and ET-1. The increase in [Ca 2+ ]i may acts as intracellular signaling in the migration in response to PDGF-BB and ET-1 in VSMCs.
