Changes of ceramide and apoptosis during myocardial ischemia/reperfusion
- VernacularTitle:心肌缺血/再灌注损伤神经酰胺含量变化和凋亡机制的研究
- Author:
Huilan SUN
;
Weikang WU
;
Hanchuan LUO
;
Tianwen LIANG
- Publication Type:Journal Article
- Keywords:
Ceramides;
Myocardial reperfusion injury;
Apoptosis;
Stress
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the change of myocardial ceramide during myocardial ischemia/reperfusion and the relationship between ceramide and apoptosis and oxidative stress. METHODS: After inducing myocardial ischemia/reperfusion (I/R) injury in mice with pituitrin (Pit), myocardial SOD activity and MDA content were measured. DNA agarose gel electrophoresis and fluorescent staining of DAPI were done to check up apoptosis. The content of myocardial ceramide (?g/kg) was measured through HPTLC and scan of thin plate. RESULTS: The myocardium of I/R model group had the phenomenon of DNA ladder. Apoptosis index and ceramide content in I/R model group were higher than those in normal control group (P
