Pathophysiologic changes of local ischemic coronary artery and cardiac muscle after ligating canine LAD
- VernacularTitle:结扎犬冠脉左前降支后局部血管及心肌的病理生理变化
- Author:
Weilai HE
;
Rukun CHEN
;
Ruyuan ZHOU
;
Shenglin GE
;
Xiaoyin CHEN
- Publication Type:Journal Article
- Keywords:
Coronary stenosis;
Endothelial cells;
Myocardial infarction;
Models, animal;
Coronary artery bypass
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To evaluate the reliability of making a research model of coronary artery stenosis and local myocardial infarction reproduced in dog by ligating canine LAD. METHODS: We disparted 30 aged healthy cross-breed dogs [(18.5?6.7) kg] into three groups. The near part of the LAD through left minimal thoracic incision was ligated to interdict 25% (group A), 50% (group B), 75% (group C) of the flux, respectively. The changes of plasma endothelium-derived factors NO, ET-1, sP-selectin and CTnT were measured before ligation and at different time points after ligation. The expression of P-selectin gene in cardiac muscle was detected by Western blotting. The segments of distal parts of the ligated LAD were cut and pathological changes of the patches of topical cardiac muscle were observed by electronic microscope. RESULTS: After ligation, NO/ET-1, P-selectin and CTnT had significant changes in group B (P
