The inhibitory effects of endostatin gene transfer on the growth of breast cancer cells in vivo and in vitro
- VernacularTitle:内皮抑素基因转移对乳腺癌细胞生长影响的体内和离体研究
- Author:
Xia BAI
;
Jianxin FU
;
Wei WANG
;
Xueshun XIE
;
Changgeng RUAN
- Publication Type:Journal Article
- Keywords:
Endostatin;
Retroviral vector;
Breast neoplasms;
Neovascularization, pathologic
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the therapeutic action of secreted endostatin (ES) on breast cancer cells. METHODS: Retroviral-mediated endostatin gene was transferred to breast cancer cell line MDA-MB-231. The ES biological properties and function were evaluated by polymerase chain reaction (PCR), MTT and a murine xenograft model. RESULTS: After retroviral transduction, endostatin genetically modified breast tumor cells were confirmed by PCR, and the integration and durative expression of endostatin gene was successfully committed. Compared with controls, endostatin secreted by genetically modified cells markedly inhibited endothelial cell proliferation (P0.05). The results of the transplanted subcutaneous tumor model in nude mice suggested that the subcutaneous growth of MDA-MB-231 was significantly inhibited by the expression of endostatin gene (P
