Anti-cancer effect of cytotoxic T lymphocytes activated by sensitized dendritic cells
- VernacularTitle:致敏树突状细胞活化细胞毒性T细胞抗肿瘤作用的研究
- Author:
Xianrang SONG
;
Ling WEI
;
Xingwu WANG
;
Xingkui XUE
;
Lihua SONG
- Publication Type:Journal Article
- Keywords:
Dendritic cells;
T-lymphocytes, cytotoxic;
Colorectal neoplasms
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the anti-cancer effect of cytotoxic T lymphocytes (CTL) activated by sensitized dendritic cells (DCs). METHODS: Immature DCs were induced in vitro from peripheral blood monocytic cells (PBMC) and sensitized by adding tumor cells antigen extract. DCs were identified by their morphology and surface markers. MTT assay was used to evaluate the killing activity of CTL activated by sensitized DCs. The effects of specific CTL cells on inhibiting transplanted tumor HT-29 growth and on preventing HT-29 tumor generation were evaluated by injecting CTL into nude mice. RESULTS: After cultured for seven days, a large number of activated DCs were obtained with typical morphology, extensive stimulatory proliferation capacity and high CD80 (63.5%), CD83 (67.6%) and CD3/HLA-DR (83.2%) expressions. The killing activity of CTL at 20∶1 ratio of effective cells to target cells was more than 75% to tumor cells, 35%-45% to homologous cell line and weaker to other germ cell line (P
