Effect of NS-398 on invasion of colon cancer HT-29 cells in vitro and its regulation by CD44v6 and nm23-H1 genes
- VernacularTitle:COX-2抑制剂(NS-398)对结肠癌HT-29细胞体外侵袭力的作用及CD44v6、nm23-H1基因的调节
- Author:
Xiaoqing JIA
;
Ming YAN
;
Fanli MENG
;
Ning ZHONG
;
Guangtao XIA
;
Yanqing LI
;
Shangzhong ZHANG
- Publication Type:Journal Article
- Keywords:
HT-29 cells;
NS-398;
Colonic neoplasms;
Invasiveness, neoplasm;
CD44v6;
nm23-H1;
Prostaglandin-endoperoxide synthase
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the anti-invasive effect of NS-398 on colon cancer cell line HT-29 in vitro an its regulation by CD44v6 and nm23-H1 genes. METHODS: Flow cytometry was used to detect the expression of COX-2 and CD44v6 in HT-29 cells. MTT was used for cell survival rate tests. The modified Boyden chamber model was used for quantitative invasion assay. RT-PCR was used to detect the expression of nm23-H1 mRNA. RESULTS: Flow cytometry analysis showed that COX-2 was positive in HT-29 cells. NS-398 had significant inhibitory effects on invasion of HT-29 cells, which had no relation with its cytotoxicity. NS-398 down-regulated the expression of CD44v6 and up-regulated the expression of nm23-H1 mRNA. CONCLUSION: NS-398 has an anti-invasive effect on HT-29 cells in vitro. Down-regulation of CD44v6 and up-regulation of nm23-H1 may be its underlying mechanisms.
