Nuclear factor-?B activation is involved in MCP-1 expression in human mesangial cells induced by Ox-LDL
- VernacularTitle:N核因子-?B活化参与Ox-LDL诱导人肾小球系膜细胞表达单核细胞趋化蛋白-1(英文)
- Author:
Huiming WANG
;
Miaozhen ZHU
;
Xiang XU
;
Jianguo ZHANG
;
Yani HE
;
Kailong LI
- Publication Type:Journal Article
- Keywords:
Lipoproteins, LDL;
Mesangial cells;
Monocyte chemoattractant protein-1;
NF-kappa B
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the role of nuclear factor-?B (NF-?B) in the expression of monocyte chemoatractant protein-1 (MCP-1) in human mesangial cells (HMCs) induced by oxidized low-density lipoprotein (Ox-LDL). METHODS: HMCs were used as target cells. Inhibitory ?B? (I?B?) and MCP-1 protein level was measured by cell ELISA. Activities of transcriptional factors NF-?B were determined by electrophoresis mobility shift assay (EMSA). Immunohistochemistry was used to detect the translocation of Rel p65. RESULTS: NF-?B DNA-binding activation in MCs was observed when 10-100 mg/L Ox-LDL was added to the medium, and 50 mg/L Ox-LDL caused the strongest effect (8.50?1.14, P
