Effects of diammonium glycyrrhizinate on the lipid peroxidation and interstitial collagenase activity in fibrotic liver in rats
- VernacularTitle:甘草酸二铵对肝纤维化大鼠肝脏脂质过氧化与间质性胶原酶活性的影响
- Author:
Chenghai LIU
;
Fenghua LI
;
Wenhui CHEN
;
Dingzong WU
;
Yiyang HU
;
Ping LIU
- Publication Type:Journal Article
- Keywords:
Glycyrrhizic acid;
Liver cirrhosis;
Lipid peroxides;
Collagenases
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of diammonium gycyrrhizinate (Ganlixin) against liver fibrosis through preventing lipid peroxidation and regulating interstitial collagenase activity. METHODS: The liver fibrotic model was induced through subcutaneous injection of CCl_4 and the feeding with high fat and low protein in rats. Diammonium gycyrrhizinate was administered (70 mg/kg rat BW). Hepatic inflammation and collagen were observed with H-E and Sirius red staining. The liver function including serum ALT, AST activity, Alb and total bilirubin levels were determined. The hepatic lipid peroxidation including SOD and GSH-Px activities, MDA and GSH content were also measured. Hepatic hydroxyproline (Hyp) content was detected with Jamall's method. The activity of interstitial collagenase in liver was assayed by the reaction with [3H] labeled type I collagen, and the gene expression of ?1(Ⅰ) pro-collagen was analyzed by RT-PCR. RESULTS: The model rats had remarkable inflammatory necrosis, collagen accumulation and fibrosis at liver, while the diammonium gycyrrhizinate treated group showed slighter hepatic injury and collagen deposition, and the much better liver function than the model. The diammonium gycyrrhizinate-treated group had lower levels of hepatic MDA, Hyp and ?1 (Ⅰ) pro-collagen mRNA expression than those in the model group, but had higher levels of interstitial collagenase activity, GSH content, SOD and GSH-Px activity than those in the model group. CONCLUSION: Diammonium gycyrrhizinate has a good effect against liver fibrosis, which may be related to the prevention from lipid peroxidation and improvement of interstitial collagenase activity in fibrotic livers.
