Characters of the fibroblast-like cells cultured from the mobilized peripheral blood cells
- VernacularTitle:从G-CSF动员的外周血细胞悬液培养的成纤维细胞样细胞的特性分析
- Author:
Dunyun SHI
;
Qiongli ZHANG
;
Yuzhu LI
;
Ming LI
;
Yun XU
;
Jiacai ZHUO
;
Xin DU
;
Mingchun WANG
- Publication Type:Journal Article
- Keywords:
Dendritic cells;
Mesenchymal stem cells;
Monocytes;
Fibroblast-like cells;
Macrophages
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the characters of fibroblast-like (F-L) cells cultured from granunocyte clony stimunating factor (G-CSF)-mobilized peripheral blood cell (PBC) harvests. METHODS: The adherent cells in the PBC harvests were cultured for 2 week in the mediums of RPMI-1640/L-DMEM/G-CSF or interleukin-3 (IL-3) plus RPMI-1640, the cultured F-L cells were analyzed by flow cytometry (FC). RESULTS: The adherent non-confluent F-L cells obtained from the four groups were similar in their phenotypes: CD33+, CD11c+, CD64+, CD14+, CD45+, HLA-DR+, CD86+, CD34-, CD38-, CD3-, CD19-, CD56-, CD29-, CD44-, CD105-. The F-L cells are similar to monocytes except CD38-and were distinct from dendritic cells (DC) or mesenchymal stem cells (MSC). CONCLUSION: The cultured F-L cells are macrophages rather than DC or MSC. G-CSF, rhIL-3 enhances their numbers.
