Effects of different concentrations of isoflurane on cerebral cortex acetylcholine content in rats
- VernacularTitle:不同浓度异氟醚对大鼠大脑皮层乙酰胆碱含量的影响
- Author:
Anshi WU
;
Yun YUE
;
Jianjing LONG
- Publication Type:Journal Article
- Keywords:
Isoflurane;
Acetylcholine;
Cerebral cortex
- From:
Chinese Journal of Anesthesiology
1997;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different concentrations of isoflurane on cerebral cortex content of acetylcholine and the underlying mechanism.Methods Six adult male SD rats weighing 300-350 g were used in this study. The guide needle was inserted and micro-electrodes were implanted 2 days before experiment. At the beginning of the experiment the micro-analysis needles were inserted into cerebral cortex and the micro-electrodes were connected to HXD-1 multi-function monitor. Micro-dialysis liquid was collected and BIS and 95% SEF were recorded before and during isoflurane anesthesia at 0.6% , 1% and 1.4% (each concentration was maintained for 30 min) and at 1 h after isoflurane inhalation was discontinued. Acetylcholine and choline concentrations in micro-dialysis liquid were determined. Results Isoflurane reduced acetylcholine and choline concentrations in cerebral cortex and decreased BIS and 95% SEF values in a dose-dependent manner. At 1 h after isoflurane inhalation was discontinued the concentrations of acetylcholine and choline didn' t return to the baseline values but the BIS and SEF returned to the baseline values. The BIS and SEF value at the disappearance of corneal reflex was 44.2 ? 2.2 and 11.6 ? 1.7 respectively.Conclusion Our study shows that the central cholinergic system is involved in the mechanism of isoflurane anesthesia.
