Effect of persistent CO_2-insufflation on mRNA expression of adhesion molecules of colon cancer cell lines
- VernacularTitle:CO_2对结肠癌细胞表面黏附分子mRNA表达的影响
- Author:
Bo FENG
;
Minhua ZHENG
;
Junjun MA
;
Yi ZHANG
;
Jianwen LI
;
Aiguo LU
;
Mingliang WANG
;
Yanyan HU
;
Jun ZHANG
;
Bingya LIU
;
Hongwei LI
- Publication Type:Journal Article
- Keywords:
Colonic neoplasms;
Laparoscopy;
Pneumoperitoneum;
Neoplasm metastasis
- From:
Chinese Journal of General Surgery
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of CO_2-insufflation on mRNA expression of adhesion molecules of colorectal cancer cell lines. Methods With an artificial pneumoperitoneum model in vitro, SW1116 human colon carcinoma cell lines were exposed to CO_2-insufflation of different pressure ( 6mmHg , 9mmHg, 12mmHg and 15mmHg) for 1 hour. The mRNA level of E-cadherin, ICAM-1, CD44 and CD44v6 were measured after 0, 12, 24, 48 and 72 hours by real-time RT-PCR. Control cell lines were exposed to room air. ResultsThe mRNA expression of E-cadherin, ICAM-1, CD44 and CD44v6 increased significantly at 0h～48h after the cells were exposed to the continuous CO_2-insufflation for 1 hour(P
