Effect of caffeic acid phenethyl ester on proliferation and apoptosis of human colorectal cancer cell lines
- VernacularTitle:咖啡酸苯乙酯对大肠癌细胞增殖和凋亡的影响
- Author:
Yujun HE
;
Baohua LIU
;
Debing XIANG
- Publication Type:Journal Article
- Keywords:
Colonic neoplasms;
Cell cycle;
Cell proliferation;
Caffeic acid phenethyl ester
- From:
Chinese Journal of General Surgery
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of caffeic acid phenethyl ester (CAPE) on proliferation, cell cycle and apoptosis of human colorectal cancer cell line SW480.MethodsSW480 cells were treated with CAPE .The proliferative status of cells was measured by methabenzthiazuron (MTT) assay. Cell cycle was analyzed by flow cytometry (FCM) . Apoptosis was detected by FCM. The apoptosis cells were detected by TUNEL staining.ResultsCAPE inhibited growth of SW480 cells in a dose-dependent and time-dependent manner. Cell G_0/G_1 phase rate increased, S phase rate decreased and cell apoptosis rate increased after exposed to CAPE in a dose dependent manner (2.5, 5.0 and 10mg/L). Apoptosis cells increased after the treatment of CAPE.ConclusionsCAPE inhibits the proliferation and induces apoptosis in human colon cancer cell line SW480.The effect mechanism is related to arrest the cell cycle G_1 and induce cell apoptosis.
