Plasmin-induced posterior vitreous detachment
- VernacularTitle:纤维蛋白溶解酶诱导兔眼玻璃体后脱离
- Author:
Ling HUANG
;
Lili WANG
;
Haiyan WANG
- Publication Type:Journal Article
- Keywords:
Plasmin;
Hyaluronogluco saminidase;
Vitreous detachment;
Disease models, animal
- From:
Chinese Journal of Ocular Fundus Diseases
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the effects of plasmin combined with hyaluronidase or hexafluoride SF6 on inducing posterior vitreous detachment (PVD). Methods Eighteen young pigmented rabbits were randomly divided into group A, B, and C with 6 rabbits in each. All of the right eyes were the experimental eyes and the left ones was the control. The right eyes in group A, B, and C were injected with plasmin 1 U, plasmin 1 U and hyaluronidase 20 U, and plasmin 1 U and SF_6 0.5 ml, respectively; while all of the left eyes underwent intra-vitreous injection with balanced salt solution 0.1 ml. The eyes were observed by indirect ophthalmoscopy, slit lamp examination, biomicroscopy, B-ultrasonography, and electroretinography (ERG) before and after injection respectively. At last, the retinal sections were examined by light microscopy, scanning and transmission electron microscopy. Results The results of scanning microscopy showed incomplete PVD in 2 (33.3%) experimental eyes in group A, and complete PVD in 4 (66.7%) experimental eyes in both group B and C, and the positive rate of PVD in both group B and C significantly differed from that in group A (P0.05). The results of transmission electron microscopy and light microscopy indicated unchanged retinal structure. Conclusions Compared with the application of only plasmin, plasmin combined with hyaluronidase or hexafluoride SF_6 can induce complete PVD more efficiently and do no harm to the retina.
