Simultaneous Determination of 2 Components in Compound Levofloxacin Spray by HPLC
- VernacularTitle:高效液相色谱法同时测定复方左旋氧氟沙星喷雾剂中2组分的含量
- Author:
Liangyong HUANG
;
Shiming DU
- Publication Type:Journal Article
- Keywords:
HPLC;
Compound levofloxacin spray;
Levofloxacin;
Ganciclovir;
Content
- From:
China Pharmacy
2005;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a HPLC method for the simultaneous assaying of levofloxacin and ganciclovir in compound levofloxacin spray.METHODS: The chromatographic column was Dikma C18 with column temperature at 30?; the mobile phase was composed of 0.025mol/L H3PO4 solution - acetonitrile(87 : 13) with detection wavelength at 278nm and flow speed at 1ml/min.RESULTS:Good linear relations were achieved when the concentration ranges of levofloxacin and ganciclovir were 12.5-4?g/ml(r =0.9 999)and 6.25-2?g/ml(r = 1.0 000) respectively, the recovery rates were 99.90%(RSD = 0.02%) and 99.96%(RSD = 0.06%) respectively .CONCLUSION: This method is convenient, fast, reproducible and with high recovery rates, which can be used to determine 2 constituents simultaneously of compound levofloxacin spray and control its quality.
