Determination of Vitamin B_1 and Vitamin B_6 in Gengnianling Capsules by HPLC Simultaneously
- VernacularTitle:高效液相色谱法同时测定更年灵胶囊中维生素B_1、B_6的含量
- Author:
Yuan HU
;
Bing WU
;
Lin CHEN
;
Youping LIU
- Publication Type:Journal Article
- Keywords:
HPLC;
Gengnianling capsule;
Vitamin B1;
Vitamin B6;
Content
- From:
China Pharmacy
2005;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determinate vitamin B1 and vitamin B6 in Gengnianling capsules by HPLC simultaneously .METHODS: The separation was performed on Hypersil-ODS C18 column, methanol - sodium hexanesulfonate solution(20 : 80) was used as mobile phase with a flow rate of 0.8ml/ min and detection wavelength of 280nm.RESULTS: Linear correlations with peak area scores were achieved when the sample size of vitamin B1 and vitamin B6 were with a range of 0.884?g-2.652?g (r = 0.9 999) and 0.714?g-2.142?g(r = 0.9 999) .respectively, the average recovery of which were 95.87%(RSD = 0.82%) and 101.96% (RSD = 0.86%), respectively .CONCLUSION: The method is simple, accurate and it can be used for quality control of Gengnianling Capsule.
