Content Determination of Gallic Acid in Tea Pigment Injection by RP - HPLC
- VernacularTitle:反相高效液相色谱法测定茶色素注射液中没食子酸的含量
- Author:
Huiyi LU
- Publication Type:Journal Article
- Keywords:
Tea pigment injection;
RP - HLPC;
Gallic acid;
Content
- From:
China Pharmacy
2005;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To develop a method for the determination of gallic acid in tea pigment injection. METHODS: RP HLPC method was adopted in which the separation of gallic acid was performed on C18 chromatographic column with a column temperature of 20℃; the mobile phase was composed of acetic acid - McOH - DMF - H2O(2 : 8 : 30 : 160) with a flow rate of 0.5ml/ mm and detection wavelength of 280nm .RESULTS: The linear range of gallic acid was 8.4-92.4?g/ ml( r = 0.9 997),the average recovery rate was 99.13% (RSD = 1.55%) .CONCLUSION: The method developed in the present study is of high specificity .sensitivity .precision,recovery and reproducibility and it can provide a basis for the formulation of quality control method for tea pigment injection.
