Content Determination of Forsythin in Qingreling Granules by Solid Phase Extraction-HPLC
- VernacularTitle:固相萃取-高效液相色谱法测定清热灵颗粒中连翘苷的含量
- Author:
Xia KUANG
;
Tai WANG
- Publication Type:Journal Article
- Keywords:
Solid phase extraction-HPLC;
Qingreling granules;
Forsythin;
Content
- From:
China Pharmacy
2005;0(20):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a HPLC method for content determination of forsythin in qingreling granules by C 18 solid phase extraction to purify the forsythin in qingreling granules.METHODS:The column of Dikma Diamonsil TM C 18 ,a mob_ ile phase of acetonitrile-water(30∶70),the flow rate of1.0ml/min,the detection wavelength of230nm,and the column temperature of30℃.RESULTS:The calibration curve of forsythin was linear in the range of10.0～200.0?g/ml(r=1.0000),the average recovery was98.1%(RSD=1.91%).CONCLUSION:The present method is accurate,reliable,simple and with good reproducibility,the sample was purified completely,and little interference and it can be used for quality control of qingreling granules.
