Test for Bacterial Endotoxins in Cefalotin Sodium for Injection by Gel Clot
- VernacularTitle:凝胶法检查注射用头孢噻吩钠中的细菌内毒素
- Author:
Ping CHEN
- Publication Type:Journal Article
- Keywords:
Gel clot;
Cefalotin sodium for injection;
Bacterial endotoxins;
Interference test
- From:
China Pharmacy
2005;0(20):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for test of bacterial endotoxin on cefalotin sodium for injection by gel clot.METHODS:Cefalotin sodium for injection was diluted in water for determination of bacterial endotoxin and allocated to sample negative and sample positive controls to determine its maximum noninterference concentration;Then bacterial endotoxins were diluted into solutions of different concentrations with the test solutions,and interference test was carried out by using tachy?pleus amebocyte lysate sensitivity compound nucleus.RESULTS:The maximum noninterference concentration of cefalotin sodium for injection was6.68mg/ml;And cefalotin sodium at the concentration of1.67mg/ml did not interfere with the test for bacterial endotoxins.CONCLUSION:The present method can replace pyrogen test with rabbit for examination of bacterial endotoxin in cefalotin sodium for injection.
