Content Determination of the Total Naphthoquinone in Oil/Water Emulsion of Shikonin by Ultraviolet Spectrophotometry
- VernacularTitle:紫外分光光度法测定O/W型紫草脂肪乳剂中总萘醌的含量
- Author:
Fangfang FAN
;
Zepu LIU
;
Wen CHEN
;
Fashou JIANG
- Publication Type:Journal Article
- Keywords:
Oil/water;
Fat emulsion of shikonin;
L-shikonin;
Total naphthoquinone;
Content determination
- From:
China Pharmacy
2005;0(20):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for content determination of the total naphthoquinone in oil/water emul?sion of shikonin by ultraviolet spectrophotometry.METHODS:The content of the total naphthoquinone was counted in L-shikonin,methanol was used as solvent and the wavelength was set at516nm.RESULTS:The concentration of L-shikonin had good linear correlation with absorbance in the range of8.32～41.60?g/ml(r=1.0000,n=5),the average recovery was98.6%(RSD=0.53%).CONCLUSIONS:The present method is simple,precise and replicable resulting in high recovery and accuracy,it can be used to determine the content of total naphthoquinone in oil/water emulsion of shikonin.
