Effect of curcumin on heme oxygenase -1 expression in the lung of rats treated with LPS
- VernacularTitle:Curcumin对注射脂多糖大鼠肺脏血红素氧合酶-1表达的影响
- Author:
Xinli HUANG
;
Yiling LING
;
Hongyan DAI
;
Chunhua DING
;
Qiuhong WANG
- Publication Type:Journal Article
- Keywords:
Activator protein-1;
Lipopolysaccharides;
Lung;
Heme oxygenase (decyclizing)
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the regulatory effect of curcumin on expression of heme oxygenase-1 (HO-1) in the lung of rat treated with LPS. METHODS: Eighteen rats were divided into three groups injected with different agents via lingua vein: control group (animals received equivalent saline) , LPS group (animals received a bolus dose of LPS 5 mg?0.5 mL-1?kg-1) and LPS+ curcumin group (animals received AP- 1 inhibitor curcumin 20 mg?0.5 mL-1?kg-120 min before the injection of LPS 5 mg ?0.5 mL-1?kg-1) . The expression of HO-1 mRNA and protein in the lung were examined 7 h after LPS administration by reverse transcribed polymerase chain reaction (RT- PCR) and Western blotting, respectively. Carboxyhemoglobin (HbCO) formation within pulmonary tissue was measured to represent CO content. RESULTS: The results showed that HO- 1 mRNA and protein expression as well as CO content in the lung of rats in LPS group were significantly higher than those in control group (P
