Effects of cardiomyopeptidin pretreatment on anoxia-reoxygenation injury to hippocampal neurons in rats
- VernacularTitle:心肌肽素预先给药对大鼠海马神经元缺氧复氧损伤的影响
- Author:
Rongguo LIU
;
Aixia HE
- Publication Type:Journal Article
- Keywords:
Peplides;
Cell hypoxia;
Neurons;
Hippocampus
- From:
Chinese Journal of Anesthesiology
1995;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of cardiomyopeptidin (CMP) pretreatment against hippocampal neuronal injury caused by anoxia-reoxygenation and the underlying mechanism. Methods Hippocampal neurons were isolated from neonatal SD rats and cultured for 9-10 days. The cultured primary hippocampal neurons were randomly divided into 4 groups: ( I ) control group; ( II ) anoxia-reoxygenation group (A-R); (I) CMP 10?g?ml-1 group (CMP1) and (IV) CMP 100 ?g?ml-1 group (CMP2). In A-R group hippocampal neurons were subjected to 4 h anoxia (cultured in 95 % N2 + 5 % CO2 ) and 48 h reoxygenation (cultured in aerobic environment). In group III and IV CMP was added to culture media with end-concentration of CMP 10 ?g?ml-1 or 100?g?ml-1 before oxygen-deprivation. Neuronal viability was assessed by MTT method and Bcl-2 protein expression was determined by immune-cytochemistry.Results The optical density (OD) value of hippocampal neurons and the Bcl-2 expression were significantly higher in group IV (CMP2) than those in group Q (A-R) and III (CMP1) (P
