Protective effect of recombioant ?-definsin-2 on the lung against acute injury induced by pseudomonas aeruginosa infection in rats
- VernacularTitle:重组?-防御素-2对呼吸道绿脓杆菌感染大鼠急性肺损伤的保护作用
- Author:
Haihong WANG
;
Qiang SHU
;
Zhuo SHI
- Publication Type:Journal Article
- Keywords:
Defensins;
Respiratory distress syndrome,adut;
Pseudomonas infections
- From:
Chinese Journal of Anesthesiology
1995;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The purpose of this study was to determine if recombinant ?-defensin-2 can prevent the lung from being injured by pseudomonas aeruginosa infection. Methods Ten male SD rats ( class SPF) weighing 250-300 g were randomly divided into 2 groups ( n = 5 each) : I defensin group and II control group. The animals were anesthetized with intraperitoneal thiopental 10 mg? kg-1 and intubated. 50 ? l of 5 ? 107 PFU? ml-1 adenovirus with or without ?-defensin-2 gene was instilled into the trachea via tracheal tube. 48 h later 200 ? l of 6? 108 CFU?ml-1 pseudomonas aeruginosa ATCC27853 was instilled into the trachea in both groups. All the rats were killed after 24 h and the lungs were removed for (1) histologic examination, (2) determination of ICAM-1 expression in the lung and (3) broncho-alveolar lavage. The number of WBC and pseudomonas aeruginosa in broncho-alveolar lavage fluid (BALF) was counted. Results The number of WBC and pseudomonas aeruginosa in BALF was significantly less in defensin group than in control group. The degree of instologic damage and the expression of ICAM-1 in lung tissue were significantly decreased in the defensin group as compared with control group. Conclusion Recombinant ?-defensin-2 can kill pseudomonas aeruginosa in vivo and modulate the expression of ICAM-1. It can protect the lung against injury induced by pseudomonas aeruginosa infection.
