The Study of Silica Nanoparticles Modified as Gene Carriers Transfected Adult Human Epidermal Keratinocyte
- VernacularTitle:硅纳米载体转染成人角质形成细胞的初步研究
- Author:
Jianda ZHOU
;
Chengqun LUO
;
Yong CHENG
- Publication Type:Journal Article
- Keywords:
Silica nanoparticle;
Gene transfection;
Keratinocyte;
Carrier
- From:
Journal of Chinese Physician
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the ability of silica nanoparticles as gene carriers for adult human epidermal keratinocytes gene transfection.Methods The silica nanoparticles-DNA conjugated with the enhanced green fluorescence protein plasmid DNA(pEGFP-N_1) was transfected into adult human epidermal keratinocytes.The expression of green fluorescence protein was investigated in transfected keratinocytes by eletromicroscope examine and the efficiency of gene transfection was revealed.Results The silica nanoparticles-DNA complexes can be effectively transfected into adult human epidermal keratinocytes and the efficiency of gene transfection was about 20%～30%.Conclusion The silica nanoparticles can be used as DNA carriers for gene transfection,and can efficiency transfect the pEGFP-N_1 into adult human epidermal keratinocytes.
