Content Determination of Serum Nifedipine by Micell Liquid Chromatography
- VernacularTitle:胶束液相色谱法测定血清中硝苯地平的含量
- Author:
Lijuan CHEN
- Publication Type:Journal Article
- Keywords:
Micell liquid chromatography;
Nifedipine;
Serum;
Content
- From:
China Pharmacy
2005;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish micell liquid chromatography for the content determination of nifedipine in human serum.METHODS:The determination was performed on C 18 chromatographic column,the mobile phase consisted of methanol-water(60∶40,which containing20mmol/L SDS),the detection wavelength was248nm,the sensitivity was0.01AUFS,the flow rate was1.0ml/min and the chart speed was2.1mm/min.RESULTS:The linear range for nifedipine was4～40ng/ml(r=1.0002),the average recovery was98.15%(RSD=1.49%).CONCLUSION:The method is simple,accu?rate,feasible,reproducible,and it can be used as a new method for the monitoring of clinic haemoconcentration of nifedipine.
