Content Determination of the Principal Agent in Bumetanide Tablets by HPLC-Fluoremetry
- VernacularTitle:高效液相色谱-荧光法测定布美他尼片中主药的含量
- Author:
Kun TANG
- Publication Type:Journal Article
- Keywords:
HPLC-fluoremetry;
Bumetanide;
Content determination
- From:
China Pharmacy
2005;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for content determination of the principal agent in bumetanide tablets by HPLC-fluoremetry.METHODS:HPLC-fluoremetry was carried out using a Kromasil C 18 column and a mobile phase con?sisting of acetonitrile-water(50∶50),the fluorescence detector of228nm for excitation and418nm for emission was used,the internal standard was dixiben,the flow rate was0.6ml/min,the sample size was20?l,the column temperature was the same as room temperature.RESULTS:The concentration of bumetanide was linear in the range of16.75～335.00?g/ml(r=0.9997),the average recovery was99.26%(RSD=0.49%,n=3).CONCLUSION:The present method is convenient and reliable,whi_ ch can be used for content determination of the principal agent in bumetanide tablets.
