Content Determination of Ursolic Acid in Pedo-stomach-invigorating&Digestion-promoting Granule by TLC-scanning Method
- VernacularTitle:薄层扫描法测定小儿健胃消食颗粒中熊果酸的含量
- Author:
Shubei WANG
;
Hengjie YUAN
;
Hong JIANG
;
Ruipeng SHEN
- Publication Type:Journal Article
- Keywords:
TLC-scanning method;
Pedo-stomach-invigorating&digestion-promoting granule;
Ursolic acid;
Content
- From:
China Pharmacy
2005;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the determination of ursolic acid in pedo-stomach-invigorating&digestion-promoting granule.METHODS:The determination was performed by TLC-scanning method in which chloro-form-acetone-glacial acetic acid(18∶2∶0.2)was used as developing solvent,and10%sulphuric acid ethanol solution was taken as developer,which were baked under110℃until limpid prunosus spots were occurred,the scanning method was dual wavelength reflection zigzag scanning with detection wavelength at520nm,reference wavelength at700nm,linearity parameter Sx=3and slit size at0.4mm?0.4mm.RESULTS:Ursolic acid has a good linear relation with peak area score when its sample size was within the range of2?g～10?g(r=0.9996),the average recovery is97.8%(RSD=1.4%).CONCLUSION:This me_ thod can be served as a quality control for pedo-stomach-invigorating&digestion-promoting granule.
