Effects of Imiquimod on the Secretion of Cytokines by Human Epidermal Langerhans Cells and Ha- CaT Cells
- VernacularTitle:咪喹莫特对人表皮朗格汉斯细胞及HaCaT细胞分泌细胞因子的影响
- Author:
Xu MIAO
;
Dan LUO
;
Xi JI
;
Lixian XU
;
Wei MIN
;
Xiangfei LIN
;
Yang XU
- Publication Type:Journal Article
- Keywords:
Imiquimod;
Langerhans cells;
Keratinoeytes;
Cytokines
- From:
Chinese Journal of Dermatology
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of imiquimod on cytokine secretions of normal human epidermal Langerhans cells (LCs) and HaCaT cells, and to better understand the mechanism of immune regulation by imiquimod. Methods LCs were sorted from prepared epidenmal cells by density gradient centrifugation and magnetic-activated cell sorting (MACS). LCs and HaCaT cells were cultured in media with or without 5 ?g/mL of imiquimod for 4 hours, then cell-free culture supernatants were harvested, cytokines were detected by ELISA kits. Results TNF-?, IL-1? and IL-6 secreted from imiquimod treated LCs were all higher than those from control LCs (all P
