Effect of respiratory syncytial virus on apoptosis and expressions of FasL, Fas, Bcl-2 and Bax
- VernacularTitle:呼吸道合胞病毒感染对细胞凋亡及FasL、Fas、Bcl-2、Bax基因表达的研究
- Author:
Huiqin PENG
;
Zhaoming YE
;
Jing QIAN
;
Jun TAO
- Publication Type:Journal Article
- Keywords:
Respiratory syncytial virus;
Apoptosis;
A549 cells;
Gene expression
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the relationship between respiratory syncytial virus (RSV) infection and apoptosis, between RSV infection and expressions of FasL, Fas, Bcl-2 and Bax. METHODS: Apoptotic cells were examined by flow cytometry and transmission electron microscope. Immunohistochemical analysis was used to detect the expressions of apoptosis-associated gene FasL, Fas, Bcl-2 and Bax in A549 cells during RSV infection. RESULTS: Apoptotic index increased at 72 h and 120 h postinfection. Apoptotic cells were detected by transmission electron microscope. High-expressions of FasL, Fas and Bax genes and low-expression of Bcl-2 gene were detected by immunohistochemical staining. CONCLUSION: Apoptosis in A549 cells was induced by RSV infection. This apoptosis may be induced by up-regulating the expression of FasL, Fas, Bax genes and down-regulating the expression of Bcl-2 gene.
