The culture and differentiation of adult bone marrow-derived pluripotential mesenchymal stem cells into insulin-producing cells
- VernacularTitle:小鼠骨髓多能成体祖细胞的分离、培养及体外诱导分化为胰岛素分泌细胞的研究
- Author:
Lihong CHEN
;
Hua CHENG
;
Jun MIN
;
Muchao WU
;
Li YAN
;
Zuzhi FU
- Publication Type:Journal Article
- Keywords:
Bone marrow;
Stem cells;
Cell culture;
Cell differentiation;
Insulin-secreting cells
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the culture and characteristics of mouse adult bone marrow-derived pluripotential mesenchymal stem cells and its potential to differentiate into insulin secretion cells. METHODS: Cells were plated on 60% DMEM-LG and 40% MCDB-201 medium supplemented with 2% fetal calf serum and 10 ?g/L PDGF-BB, 10 ?g/L EGF and 1?10~6 U/L LIF. The proliferation rate, phenotype and oct-4 mRNA were tested. After it was plated on serum-free medium DMEM/F12 with GLP-1 and nicotinamide, the nkx2.2 ngn3, pdx-1 and insulin 2 mRNA were tested. RESULTS: The cells were round with large nucleus and scant cytoplasma. They were CD13~+, CD44~-, CD45~- and MHCⅡ~-. Oct-4 mRNA were present. The nkx2.2 pdx-1 and insulin 2 mRNA were presented in cells plated on the inducing medium at 14 days. CONCLUSION: The adult bone marrow-derived pluripotent stem cells were cultured and they has the possibilities to be induced into insulin-secreting cells.
