Decreased fibroblast proliferation and collagen content in the wound of diabetic rats caused by streptozotocin
- VernacularTitle:糖尿病大鼠创面愈合过程中成纤维细胞增殖及Ⅰ型胶原合成减少
- Author:
Ping LI
;
Guangshan LI
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus;
Wound healing;
Fibroblasts;
Collagen
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study fibroblast proliferation and collagen synthesis during wound healing in diabetic rats induced by streptozotocin. METHODS: 30 Wistar male rats were randomly divided into control group and model group. 55 mg/kg STZ were given intraperitoneally to model rats. After 3 weeks, a round skin of 2.04 cm~2 was excised on all dorsal back of rats. The healing time and healing rate were observed according to re-epithelization. The numbers of fibroblasts and the expression of proliferating cell nuclear antigen (PCNA) were observed by Hematoxylin-Eosin (HE) staining and immuno-histochemistry assay. Collagen Ⅰ and Ⅲ stained by Picric acid-Sirius red were calculated by image analysis. RESULTS: The healing time in model group was (27.13?1.81) days,significantly longer than that in control group [(15.25?1.67) days, P
