Role of injury and phenotype shift of liver sinusoidal endothelial cells in the development of portal hypertension of cirrhosis in rats
- VernacularTitle:肝窦内皮细胞损伤和表型改变在大鼠肝硬化门脉高压发生中的作用
- Author:
Xianbo WANG
;
Ping LIU
;
Zhipeng TANG
;
Xiong LU
;
Chenghai LIU
;
Yiyang HU
;
Lieming XU
;
Hongtu GU
;
Cheng LIU
- Publication Type:Journal Article
- Keywords:
Rats;
Liver cirrhosis;
Nitric oxide;
Endothelins;
Hypertension, portal
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the role of injury and phenotype shift of liver sinusoidal endothelial cells in the development of portal hypertension of liver cirrhosis in rats. METHODS: The rat liver cirrhosis model was established by peritoneal injection of dimethylnitrosamine (DMN) (at a dose of 10 mg?kg~(-1), 3 times a week, for 4 weeks). The dynamic changes of liver cirrhosis were observed at different time points (1 day, 2 days, 3 days, 1 week, 2 weeks, 4 weeks, 6 weeks and 8 weeks). The pressure of portal vein (Ppv), the expression of CD44, von Willebrand factor (vWF), endothelin-1 (ET-1) mRNA and endothelial nitric oxide synthase (eNOS) mRNA, the serum hyaluronic acid (HA) content and liver ET-1 content were measured. RESULTS: Compared with the normal control rats, CD44 positive staining was weak in the 1 day model rats, and the numbers of fenestrae of sinusoidal endothelial cells (SECs) rapidly decreased, but serum HA content rapidly increased (P
